For your convenience, Active Motif offers Ready-to-ChIP Chromatin. Using pre-made chromatin with our chromatin immunoprecipitation (chromatin IP) kits and controls ensures that all components are confirmed to work in ChIP. So, you can more easily validate your own antibodies and primer sets.
Ready-to-ChIP Chromatin has been optimally sheared by sonication and validated in ChIP. It can be used in conjunction with all of theChIP-IT® Kits, so you can be certain that the only variable in testing an antibody for ChIP is the antibody itself.
However, in the event that Active Motif does not offer Ready-to-ChIP Chromatin from the cell line that you wish to work with, we recommend our EpiShear™ Sonicator and ChIP-IT® Express Shearing Kit, which were designed to work together when preparing sheared chromatin for ChIP.