Applications
ApplicationNotes
ValidatedApplications:
DB:1:1,000-1:10,000dilution
PublishedApplications:
ICC/IF
Seereferencesformoreinformation.Individualoptimizationmayberequired.
Immunogen
This3-methylcytosineantibodywasraisedagainst3-methylcytidineconjugatedtoKLHandrecognizes3-methylcytosine.
Buffer
Rabbitserumcontaining30%glyceroland0.035%sodiumazide.Sodiumazideishighlytoxic.
References
3-Methylcytosine(3-mC,3-methylcytidine)antibodytestedbydotblotanalysis.
BSAconjugatednucleosides(startingat10ngasindicated)werespottedontoPVDFmembraneandblottedwith3-methylcytidineantibodyatadilutionof1:10,000.
Lane1:3-methylcytidine.
Lane2:Cytidine.
Lane3:5-methylcytidine.
Lane4:5-hydroxymethylcytodine.
Background
MethylationofDNAcanoccurnon-enzymaticallyatthenitrogen-threeofthecytosinebasethroughspontaneousexposuretoendogenousS-adenosylmethionine(SAM).Theresulting3-methylcytosine(3-mC)ismutagenicandmustberepaired,whichoccursinhumansthroughthebaseexcisionrepair(BER)ordealkylationviahumanhomologuesoftheE.coliAlkBprotein.3-methylcytosineispresentinhumancelllinesandincreasedlevelsof3-mCimpairproliferation.
CompatIBLeSecondaryAntibodies
ActiveMotifhasdevelopedavarietyofhigh-qualitysecondaryantibodiesformostapplications,withanti-rabbitandanti-mouseconjugatestoabroadlineofhigh-qualityfluorescentmolecules,aswellashorserADIshperoxidase(HRP).VisitourSecondaryAntibodyConjugatespagetofindtheperfectsecondariesforimmunofluorescenceexperimentswith3-Methylcytosine(3-mC)antibody(pAb).
Storage
Someproductsmaybeshippedatroomtemperature.ThiswillnotaffecttheirstABIlityorperformance.Uponreceipt,unconjugatedantibodiesmaybestoredat-20°Cforupto2years.Fluorophore-&enzyme-conjugatedantibodiesshouldbestoredat4°C.Fluorophore-conjugatedantibodiesshouldbeprotectedfromlight.Keepreagentsonicewhennotinstorage;toavoidrepeatedfreeze/thawcycles,werecommendaliquotingitemsthatwillbestoredfrozenintosingle-usefractionspriortofreezing.
Guarantee
Thisproductisguaranteedfor6monthsfromdateofreceipt.
Thisproductisforresearchuseonlyandisnotforuseindiagnosticprocedures.
ApplicationKey
- ChIP=ChromatinImmunoprecipitation;
- DB=DotBlot;
- ELISA=Enzyme-linkedImmunosorbentAssay;
- EMSA=ElectrophoreticMobilityShiftAssay
- FC=FlowCytometry;
- ICC=Immunocytochemistry;
- IF=Immunofluorescence;
- IHC=Immunohistochemistry;
- IP=Immunoprecipitation;
- MeDIP=Methyl-DNAImmunoprecipitation;
- TR-FRET=Time-ResolvedFluorescenceResonanceEnergyTransfer;
- WB=WesternBlotting
