The ChIP-IT^® qPCR Analysis Kit was designed to simplify ChIP qPCR analysis and data interpretation. The kit includes standard curve DNA, a standard curve primer pair, positive and negative control qPCR primer sets for human and mouse samples and an analysis spreadsheet that automatically normalizes and graphs the data. The normalization calculations take into consideration the amount of chromatin that was used in the ChIP reaction, the resuspension volume of the ChIP DNA and the primer efficiencies to introduce a level of consistency in the data output that enables a reliable comparison of many samples across multiple experiments. Based on the consistency of the data output, Active Motif is able to make specific recommendations in regards to data interpretation so that customers can reliably assess the quality of their ChIP DNA. We recommend using Active Motif"s ChIP-ITHigh Sensitivity^® Kit to perform the chromatin immunoprecipitation reactions.

Please click on the link to download a copy of the ChIP-IT^® qPCR Analysis Spreadsheet.